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written by reader Of Needles, Haystacks, and the Needle-in-a-Haystack Cancer Diagnostics Nanocap That May Belong in Your Portfolio

By DrKSSMDPhD, June 11, 2014

[Ed. Note: Here is the latest from Dr. KSS, who writes about health, medicine and biotech stocks for the Irregulars. He has agreed to our trading restrictions, and his words and ideas are his own.]

Dedicated to Cynthia Chew Choon Hoon (1959-2013). In pace requiescat.

Imagine a day down the hallway of the future. You’re a happy Gumshoe Irregular, you’ve just turned 65, and last week, you had a routine check-up with your doctor. You feel great and are looking forward to a contented, ebullient retirement. It’s early evening. You and your wife are having gin-and-tonics on the veranda, watching the sunset. The lime in your drink is tart, zesty, and life is good.

But the phone rings. Your wife answers, ignoring your importunings that she ignore it. She beckons you. It’s your doctor, and he wants to speak with you. Can’t be good.

The doctor is still in his office going over labs and test results, and decided to call you rather than sending a letter. Last week, he drew a PSA and also examined your prostate. It seemed fine. But it’s your PSA that concerns him. It is now 4.5, not screamingly high, but up from last year’s level of 4. He wants you to come in again.

You imagine a device being shoved into your rectal vault, from which lots of needles will suddenly extrude and plant themselves painfully deep into your prostate gland, translocating germs from your rectum into it, causing bleeding for days on end…and you begin feeling dizzy. Your ears begin to ring, you break out into a cold sweat. Your worst fears are coming true, you begin seeing spots, and slide onto the floor. Little birds fly around your head.

“Hon?! Are you OK?,” your wife shouts.

“Hello……sir? Are you still there?” the doctor is shouting into the phone.

Your wife grabs the phone. “Doctor, my husband just fainted!”

“Is he OK?”

“Just dizzy and sweaty. He’s coming around.”

“I must have scared him. I told him his PSA is high.”

“Yeah, he mumbled something about prostate biopsies? I think that put the fear of God into him.”

“Prostate biopsies? I didn’t say anything about that.”

“Isn’t that what you are planning doctor?,” your wife asks.

“Well, no, not at all. I just wanted him to come back so we could draw blood, you know, to look for any prostate cancer cells. Prostate biopsies? No, we don’t do those anymore.”

Medical science fiction? Probably not. The lab test in question, looking at blood to find circulating tumor cells, is now making its first forays into prime-time, though as of yet it is to assess response to cancer treatment rather than in place of tumor biopsies. But that is likely to change, and in fact one tiny biotechnology company seems uniquely poised to benefit from this new science and this emerging “hot”new diagnostic trend.

Do you remember this scene from The Silence of the Lambs? Have a look. Clarice Starling is wrestling with the identity of a killer, and Hannibal Lecter, trying to act as her mentor, encourages her to think like Marcus Aurelius and mull the essence, the nature, of the killer.

Cancer is a killer. But what is cancer, in its essence? What is the nature of cancer? What distinguishes cancer from all other illnesses? What does cancer do that no other disease does? There’s an old word I love, quiddity, sometimes defined as that which answers the question, quid est? What is the quiddity of cancer?

Cancer spreads. It metastasizes. That is the most scary aspect of its nature. Cancer is like a flea that bounds from place to place in the body, and takes up residence easily at sites remote from where it started. Yes, cancer can extend locally, but it loves to end up at distant sites without leaving any crumbs for how it got there. How does cancer get to those places? Some will answer via the lymphatic system….that cancer goes to lymph nodes and from their more broadly into the body. But there is a better answer.

The fact is, cancer cells get into your bloodstream, and course around as if they were river rafting. And this idea is not a new concept. When they reach a rivulet, a capillary, a tight spot from which they can flow no further, they lodge. They take root there. Most of these attempts at colonizing, at metastasizing, provoke immune reactions that are deadly to the tumor cells.

Armand Trousseau (1801-1867): A Gregarious Master Clinician

Nineteenth-century France gave the world many fine physicians, including Dupuytren, Duroziez, and the astonishing Rene Laennec. But none were more remarkable than Armand Trousseau, an affable Paris internist.

On 27 June 1867, Trousseau died…to the shock of friends, patients, colleagues, seemingly to the surprise of everybody but one person: Armand Trousseau. He was 65. In January of that year, he had noticed something amiss in his left upper arm. One imagines him at a wash basin with a straight razor shaving around his bushy sideburns, and feeling a pang. He noticed a painful, tender, hot mass in his arm, a blood clot. The next day it had subsided and the mass and pain were gone. He knew what was coming, and knew he had at most 6 months.

trousseau2

The eminently likable Armand Trousseau, MD. Il a découvert et puis il l’a tué

Trousseau had many fine accomplishments—better treatments for COPD, malaria, pleuritis, goiter, yellow fever; he performed the first tracheotomy in France—and was also famously known as a patient advocate and a person who became so absorbed in listening to and studying patients at their bedside that he lost track of time. In Irving Stone’s fine historical novel about Sigmund Freud, The Passions of the Mind, he depicts a scene in which, at a teaching hospital bedside, the great Charcot extols the virtues of “see-ing,” of really opening one’s senses and studying patients, of even ignoring one’s education somewhat and letting bedside findings, primary observation, guide you in formulating disease and its treatment. Paracelsus admonished physicians always to let patients be their true textbook, that observing patients never deceives. Trousseau paid attention to patients, and little escaped his notice.

the passions of the mindIn Choruses From “The Rock,” T.S. Eliot asks, “Where is the wisdom we have lost in knowledge?” Trousseau would have sympathized with this, because regardless of his learning, regardless of what he had read, he always noticed the details of what he observed with every patient. “Get the patient well!” was what Trousseau always told medical students, and it was with patient centeredness in mind that he noticed a process no one else had: the phenomenon of migratory thrombophlebitis, later known as Trousseau syndrome. Trousseau noticed that certain people would have acute venous thrombosis with local inflammation in a limb, with disappearance of the process one or two days later, and that patients always died within 6 months of a visceral cancer, malignancy somewhere in the abdomen. He hypothesized that cancer somehow distorted the clotting system, favoring thrombosis (he was correct…except that rarely prostate cancer favors a bleeding tendency). Later observers also noticed that lung cancer causes Trousseau syndrome. And in fact, pace Trousseau, patients who have an unexplained episode of deep vein thrombosis need underlying cancer excluded. (Trivia question for medical readers: what is the Trousseau sign?)

Trousseau was among the earliest popularizers of bedside, patient-based teaching. When he had interesting or complex patients, he would summon students and trainees for long, impromptu sessions in which they gathered around the patient. Trousseau would hold forth, and recite the patient’s history with a novelistic richness, a raconteur’s vim and vigor. This often charmed patients, that he had gone to such pains to know their histories in depth. And he often amazed patients with insights about their lives, their motivations, their relationships….things they recognized as true, but had not recognized before. Many physicians who love teaching at the bedside—and I am one—love it for one reason: the teacher gets to midwife the forging of new neural links, new understanding….gets to be present at the birth of knowledge. Students are asked what they see, and how that may relate to something else they’ve seen. With the right Socratic bent, five minutes at the bedside can teach a student vastly more than six weeks at the books can, but also tends to do so with a suddenness that thrills. The ice floes of book understanding break up, and the white water of real comprehension flows. The student gets a look on his or her face suggesting that serotonin, dopamine, glutamine and norepinephrine are flooding their synapses in unison: they get it now, they never got it before, and they will never forget it. You don’t do this so you can take credit for it, and in fact you do not care if the student even remembers that it was you who helped them learn. You just want to be there for the epiphanies, the fleeting moments when a student’s eyes, brow, and lips convey that they just underwent a cognitive firestorm, and that several brain neurons that had never met are now hopelessly, inextricably intertwined.

At the height of his reputation, Trousseau delivered a fine lecture in Paris on the phenomenon of migratory thrombophlebitis, which he called phlegmasia alba dolens:

“I have long been struck with the frequency with which cancerous patients are affected with painful edema in the superior or inferior extremities, whether or not either was the seat of cancer. This frequent concurrence of phlegmasia alba dolens with an appreciable cancerous tumor led me to the inquiry of whether a relationship of cause and effect did not exist between the two, and whether the phlegmasia was not the consequence of the cancerous cachexia.” (translated from the French)

An autopsy on Trousseau showed he had advanced gastric cancer. The jovial, inspiring doctor was dead. He is still quoted in medical training programs all over this planet for his shrewdest utterance: “utiliser de nouveaux médicaments rapidement, alors qu’ils travaillent encore” (“Use new medicines quickly, while they still work.”) Even before the days of Big Pharma and vaunted claims for new medicines, Trousseau was aware of the meta-placebo effect, placebo by proxy: that sometimes newly-released medicines work, or seem to, simply because physicians have great faith in them.

Theodor Billroth, the famed Prussian-Austrian surgeon who was a contemporary of Trousseau, would go on to identify pancreas cancer cells rarely found embedded with clumps of platelets in the capillaries of pancreas cancer patients. Billroth never published this, but noted it in his personal papers. One wonders if Billroth, who advanced the art of visceral surgery considerably, could have saved Trousseau. Billroth was a personal friend of Johannes Brahms, an excellent musician, and often proofread and helped Brahms rehearse his pieces as he was writing them. Brahms would go on to die of a mysterious jaundicing illness that baffled Billroth, and about which I have long meant to publish a theory.

billroth

Theodor Billroth (1829-1894). Before there were Billroth-I and Billroth-II procedures, there was Billroth the man. He performed the first successful gastrectomy for gastric cancer in 1881.

Two years later, in 1869, Thomas R. Ashworth, a Melbourne physician, would publish the literature’s first description of circulating tumor cells in Australian Medical Journal. Ashworth had a patient who died of advanced cancer, and at autopsy found occasional cells in the patient’s blood identical to those in the cancer.

Black swans, hens’ teeth, four-leaf clovers…. and black cats in coal cellars

It’s not clear that Ashworth knew of Trousseau syndrome, or would have appreciated what would later be recognized (vide infra), which is that tumor cells trigger platelet aggregation and that this is responsible for what Trousseau observed. But like many Australians, he was clever: tumors did not metastasize via a Star Trek transporter (or choose a 19th century analogy). Best to go looking for individual tumor cells, then, loose and afoot in the system. He found them….cells in the blood identical to cells in the tumor. Looking for them was like looking for a black cat in a coal cellar. One of the ironies of Ashworth’s description of the patient and the cells is that no one has ever known for sure what kind of cancer the patient had. Ashworth described subcutaneous nodular cancer, and many feel he may have been reporting a case of soft-tissue sarcoma.

White peacocks, red bananas, insanely big crabs. Australia has always given the world rare things, like the discovery of circulating tumor cells. One thinks of black swans as rare, until you cross the Hobart, Tasmania, bridge over a waterway inhabited by them!

When cells grow in organs, they do so in an orderly, non-aggressive, well-behaved way, not violating boundaries, displaying inhibition at contact with neighboring cells. Cancer cells do not scheme badness, do not plot ways to subvert the system. As we are all of us evolved, so cancer represents certain elemental steps that go on in evolution: DNA occasionally rolls the dice, rearranges itself. Occasionally certain rules get subverted. Humans fancy the ideas of immortality like the gods have. And so it, seems, maybe do cells. They stumble upon ways to live forever, and to propagate themselves by growing aggressively and even escaping their confines, becoming flotsam and jetsam that catch blood currents to other locales. It’s not that the cells go rogue and plan to cause trouble. But gene rearrangements and mutations confer properties on cells that harm the organism, and when enough errant properties, including the abilities to invade, metastasize, repel the immune system and resist intrinsic mechanisms of apoptosis come together, the result is cancer and it kills. Humans long for, dream of, immortality. Is there a reason our cells should not do the same? (And is it possible that our craving for immortality is hubristic because it is harmful to some greater system (Jung called it the Collective Unconscious), just as cancer harms us, that we comprise but know only through-glass-darkly?)

If you have cancer, how likely are you to have circulating tumor cells? No one knows. In fact, this is a question today’s featured company may help medicine answer. Certainly the more advanced, the larger, the more inclined to metastatic spread a tumor is, the likelier it is to have breakaway stray cells found in your blood. The number of cells in a cancer is proportionate to the volume of a tumor. From geometry, we know that the volume of a sphere rises as a function of the radius to the third power. Which means that when a tumor doubles its diameter, it has 8 times as many cells as it did at its former size. So the number of circulating tumor cells should be strongly linked to tumor size, and in fact a test for circulating tumor cells as a global screening test for any kind of cancer as part of a wellness check is unlikely to happen. We will always need to continue to pay attention to organs—-breast, prostate, colon, the lungs of smokers, the skin of the Celtic and the immunosuppressed—-known to be trouble spots.

Here are some number to give you a sense of scale for the prevalence of circulating tumor cells. One liter has one million microliters, or 1000 cubic centimeters. One cubic centimeter is one milliliter. Blood has roughly one million red blood cells per microliter. Blood may have about 1000 white blood cells per microliter. But in a cancer patient, blood may have only 100 to 1000 circulating tumor cells per liter; that’s one circulating tumor cell for every one to 10 cc’s of blood.

circulating tumor cell

Technology makes it easy to spot this circulating prostate cancer cell in blood. T.R. Ashworth had no technology…it was Australia 1869 for him. (From Genelux.com)

Huahsi Night Market (華西街夜市)

In 1991, I packed and departed for Taipei. Circulating tumor cells have long fascinated me, and I was intrigued by data that circulating tumor cells often break loose only to provoke platelet aggregation around them. The tumor platelet clump may form a bigger vessel for a voyage, and once it lights or lodges at some point, the platelets release molecules that act as growth factors, fertilizer to jazz up tumor growth. I wanted to study this further, and in fact had heard of a viper unique to Taiwan with a venom component that seemed able to halt the process.

Taipei has many fine night markets, but the one at Huashi is notorious. Handlers taunt and irritate cobras while you watch, take extraordinary risks with them, and then kill them, slick them open with a knives or scissors, and empty the snake’s bile and blood into a glass of strong liquor (ask Irregular Eddy to tell you about the vaunted properties of this cocktail). I did not handle any snakes in Taiwan, but we relied on handlers like these to provide the venom for our studies.

huahsi

Huashi Night Market, Taipei, Taiwan. “Snake Alley.”

We were able to demonstrate that when we grew human breast, prostate or colon cancer cells in culture, and then made a suspension of intact tumor cells, those tumor cells would immediately provoke full aggregation, or clumping together, of platelets. When blood clots, there are two waves of it: primary hemostasis, in which a platelet plug forms at a site of injury, and then secondary hemostasis, in which (as per the article on Regado) insoluble fibrin is formed from soluble proteins in blood. Normal cells do not cause platelets to aggregate. Most tumor cells do, and with considerably potency. What’s more, the avidness with which tumor cells provokes platelets to aggregate, we showed, is proportional to how much metastatic potential they have.

What Trousseau was observing, in fact, was circulating tumor cells (some, but not all) causing tumor-cell-induced platelet aggregation. These clumps would lodge, set off an inflammatory process, but also because not enough molecular stimuli were present to provoke a full and thick clot, would probably set into motion clot-dissolving machinery (fibrinolysis), and the tumor cell/platelet clump break up after 1-2 days.

Tumor cells appear to express uncommonly high levels of tissue factor. As in the Regado article, this is a cofactor for factor VII, and when these two merge, they afford thrombin. Although collagen, epinephrine, ADP and thromboxane are platelet agonists, thrombin is the physiologic platelet agonist, and triggers platelet clumping even when present at concentrations too low to cause fibrin clot formation.

Cancer: Metaphysics, Semiotics, and Metaphors

In the late 90’s, I found myself late one summer in a situation I am sure other readers have. I was exhausted from working hard. I had a vacation week coming, but somehow had planned nothing. But I really needed to do something fun and diversionary. At the very last moment, I booked bargain passage to Budapest, seemingly the last quarter of Europe I’d not seen. I had literally packed for it by throwing things into a knapsack while a cabdriver was outside angrily blowing his horn. I was in a state of physical depletion when I left, so much so that upon renting a car in Budapest, I pulled over in a public park, stretched out on a table, and dozed off and began dreaming. I woke up feeling good, and drove off in earnest toward Slovakia, into a park near the magnificent Aggtelek Cave system, and found a wooden cabin. In chill mountain air, I turned on an incandescent lamp at the head of the bed, reached into the knapsack and fished out two books I had long meant to read: Susan Sontag’s Illness as Metaphor and AIDS and Its Metaphors. I didn’t doze off again til dawn.

Illness_as_Metaphor_(Sontag_book)

Sontag wrote the first of these books in 1978, when she was being treated for breast cancer. She deeply explored the metaphorical meaning then attached to cancer, which at the time was that somehow cancer patients had brought the illness upon themselves as a consequence of pent-up, anxious, fretting, repressed personalities. Psychiatrists attached themselves to this belief system, advocating psychotherapy to disabuse patients of the mindset, the hang-ups said to have led to their cancer. All one needs to do is spend some time at a cancer center (I formerly was on the faculty of MD Anderson Cancer Center) and you realize: this is an illness that strikes anybody, everybody, and there but for the grace of…

We know now this was nonsense, that cancer is a set of errant molecular events with no more moral or mental dimension than a head cold. You would not see a priest for pneumonia, so why see a psychiatrist for cancer? And yet even in our time there are those that still attach metaphors to cancer, often metaphors that stand to advance, to be in service of, other belief systems. I have had deeply religious patients tell me that ministers are trying to draw them deeper into the religious fold, that they’ve been told that unconfessed sin is manifesting itself as their cancer. Those same patients have been propositioned by the execrable practitioners of vitamins, herbs and the alternative, with claims that the illness owes to a lack of naturalness, to vitamin deficiencies, to quaint “imbalances” and that they know solutions (solutions from which these advocates always stand to profit). Mantra recitations do not cure mutations.

As Sontag identifies, other metaphors, still deeply trenchant, have attached themselves to tuberculosis and to HIV. I worry there may be readers here who have cancer, or who will face a diagnosis of it, who view this as the final horrifying intimation of mortality, that a cancer diagnosis is evidence of a body giving up, of a system whose machinery has gone irrevocably haywire, and that things will never be the same. Don’t. In fact, every day you are alive, somewhere in your body, you have cells undergoing transformation to cancer, ones that are conquered by various mechanisms (immune kill, apoptosis). That one of these improbable events serializes and compounds itself….these are, chances have it, things that will befall all of us given world and time enough. Anticipate. Expect. Screen. Pay attention. If and when it appears, play to win and take no prisoners.

In the threads we have discussed personalized medicine, the idea that your genes define you and provide all one needs to know about you. I am a dissident of this view: not all genes are expressed, and even for those that are expressed, we are only now in our earliest stages of understanding what turns expression of those genes on and off. This is the field of epigenetics. What is a four-dimensional printer? It is a three-dimensional printer that in hidden ways confers on the three-dimensional product attributes that allow the printed product to take on structural attributes not strictly reckoned by its three-dimensional printing. In a way, the genome is a 4-D printer: it makes a stew of proteins and RNA, and somehow that stew rises above the DNA sequence and controls it, decides what will be expressed, how and when. We are several Nobels away from a full sense of the science of epigenetics. For eons, people have argued genes versus environment, nature versus nurture. But the truth is it is neither, and both: the products of the genome, in the context of environment (temperature, nutrients, chemicals, and maybe softer constraints, such as social issues), have an amazing way of regulating the genes and how they genes behave. It is neither nature nor nurture. It is both nature and nurture, acting not as independent forces but in combined ways we don’t fully understand. It is what happens at the mysterious intersection of genes and environment that defines us. For many psychiatric illnesses, we now have a sense that it may not be a gene, and may not be a traumatic experience, but may be that the traumatic experience has permanently affected the expression of a gene. Which means that neither psychotherapy nor traditional psychotropes may help it.

trading places

In the comedy Trading Places, the fates of an investment banker and a street criminal are swapped to settle a nature versus nurture bet between two rich addle-brained aging brothers. Both lose out, in poetically just ways.

When a lump is found in a breast, when a PSA is sky-high (not 5 but, say, 50), when masses are found at colonoscopy, biopsies are done, tissue is obtained. Why?

That may seem a dumb question. The old answer was: to establish or exclude the presence of cancer. That age may be passing, however. Multi-detector CT imaging, now four-dimensional (the fourth dimension being temperature), already provides so much data corroborative of cancer that biopsy may be superfluous. A former colleague of mine, a radiologist, has become literally a chemist: he is a very bright man, and is actively developing a novel new set of radiologic imaging agents that have such unique ways of binding to tumors (and binding only to those tumors) that they may lead to imaging techniques that totally remainder the need for biopsy. For years I have had recurring arguments with oncologists over one situation: that of the cirrhosis patient with a 5 per cent per year risk of developing hepatocellular carcinoma. The patient’s alfa-fetoprotein is now 1000 (and his testicles are normal), and there is a mass that enhances in the arterial phase of a contrast-enhanced CT scan. If a biopsy of such a mass showed no cancer, would that mean anything? Would you trust that negative biopsy? I say “of course not!” You only do a test when the result will affect patient treatment. And I say, in fact, that we subject that patient to danger by sticking a needle into a safely encapsulated, walled off tumor, spewing its cells everywhere.

There is some necessity of biopsy, of course. As a medical student, I was peripherally involved in the case of a man who had an large opacified mass in his right lung. The mass could not be reached bronchoscopically, but he was bronchoscoped anyway, to look for other evidence of cancer. Washings suggested cancer cells though no cancer was seen. The patient went to the operating room, and his right lung was removed. When that lung was breadloafed up later by a pathologist, the mass proved to be a giant fungus ball, and there was no cancer. The cancer had been in the patient’s left lung, not yet apparent on CT.

A more modern reason for doing a biopsy is the personalized medicine thing: not to make a diagnosis of cancer, but to extract DNA from the cancer, and find what genes, what markers, are present, so as to guide chemotherapy. But in one of the threads, I argued that this has its problems too, and provided references. Tumors are famously heterogeneous, and a needle may have been stuck into a part of the tumor where cells simply lack the markers that will most define the overall cancer’s response to chemotherapy.

But some data, new and very recent, suggest that if one looks at circulating tumor cells, one may get the best reveal. It’s like not looking for Hollywood stars on the internet, but going straight for the Walk of Fame. The “baddest,” the most ill-behaved, most metastatic, most aggressive of the cells in a tumor may be those that pop off into the circulation.

Which is where an interesting new company comes in…

Biocept (BIOC) is a tiny San Diego company ($23 million market cap) that went public on 5 February 2014 with 4.5 million shares. I have taken a position in this company, as risky as it admittedly is, because I regard its circulating tumor cell detection methodology as best of breed.

Biocept has a proprietary method for detecting rare circulating tumor cells using a combination of multiple monoclonal antibodies (with a final avidin-biotin binding step) and complex microfluidics. It does have competition. Janssen Diagnostics (a unit of Johnson and Johnson) has a competing methodology, its so-called CellSearch machine, which several large national reference labs own. An excellent recent study, done admittedly by Biocept, compares Biocept’s method head to head with that of Janssen for detecting breast cancer cells. I have read the full text of this article, and am persuaded that Biocept’s method is superior. It claims an overall detection rate of 89 percent, versus 69 percent for Cellsearch, but for certain specific types of hard-to-find cells, Biocept’s is three times likelier to pick them up. And a success for Biocept in this arena is likely to make the stock catch fire, while broader implementation of CellSearch machines is unlikely to budge JNJ share price. I am long BIOC and have no position in JNJ.

Where Biocept’s method really outshines Janssen’s however, is as regards characterizing the cells it detects. FISH analysis is fluorescent in situ hybridization. Since a strand of RNA or DNA only binds to a complementary strand, it is possible to “probe” a cell isolated by Biocept’s method using diagnostic nucleic acid pieces, look for the presence of oncogenes.

HER2 is an important cancer marker found in about 20 per cent of breast cancer biopsies. The presence of HER2 strongly influences choice of chemotherapy. Women with HER2-positive tumors need therapy with Herceptin, a monoclonal antibody. Biocept published a fascinating study in 2013. Tumors are heterogeneous, and Biocept has evidence that a sizable number of women with HER2-negative tumors by biopsy actually have HER2-positive cells disseminated from the tumor in circulation. This is revolutionary, as those women require and will live longer with Herceptin-based regimens, even though their old-fashioned tumor biopsies said Herceptin would be neither helpful nor necessary. And there is an inherent logic that these cells would be the ones that make in into circulation: HER2 confers aggressiveness and metastatic potential on breast tumors. HER2 is a receptor tyrosine kinase related to the epidermal growth factor receptor.

Biocept is actively developing validated tests for lung cancer, prostate cancer, melanoma, and GI malignancies. Biocept’s methodology is sweetened considerably by the fact that in many aggressive tumors, in the rare cases that circulating tumor cells cannot be found, circulating tumor DNA can be found. Its methodology can detect that, something CellSearch’s cannot do. Biocept’s CEE (for cell enrichment and extraction) can sniff out tumor DNA in a sample, and probe that for tumor markers.

These are the kinds of tumors Biocept’s method can help diagnose and guide therapy for, arranged in order of deadliness:

Cancer typeDeaths/YearNew Cases/YearPeople living with diagnosis
Lung159,480228,190399,431
Colorectal50,830142,8201,154,481
Breast39,620232,3402,829,041
Prostate29,720238,5902,617,682
Melanoma9,48076,690921,780

These are statistics about Americans.

Are there risks to being in Biocept? Certainly there are, and this investment is not right for the intensely risk-averse or for a sizable part of your portfolio. My personal view is that its growth prospects are enormous, even extraordinary, even though I rarely invest in diagnostics plays. The technique is new, the methodology the company owns is definitive, and Janssen seems not intent to compete. I feel diagnostic testing with this system will be standard of care within 5 years. But herein lie risks. One is that the FDA has not approved these tests, simply because the field is too new. Approval…for what? For making an initial diagnosis? In lieu of biopsy? As a means of monitoring response to chemotherapy? The method is too new for the company even to know for what it should seek approval from the FDA. The company has many collaborative investigative arrangements with many American institutions, including MD Anderson. Meanwhile, I have spoken with the company and it is quite clear that insurers are reimbursing it for these tests, done at its facility in San Diego, because such testing is preferable to and cheaper than repeat biopsy to guide therapy. So many global institutions now have home-grown studies of ways to pick up circulating tumor cells and looking into their relevance, there is definitely the possibility that some other biotech could yet find a better method. Having read about the methods, however, I am inclined to doubt the would-be competition.

You probably have many questions.

(1) Will Biocept’s method ever totally replace tumor biopsy? That’s too early to call, but it seems probable it will, at least in some cases. The sensitivity of the method is quite high. The company will have to do studies to document the presence of circulating tumor cells in a variety of cancers and get a better sense of how advanced the cancer has to be before it can be detected in blood.

A close friend in Asia recently lost a non-smoking sister, with young children, to lung cancer. Although radiographic evidence screamed that she had cancer (she had a routine chest x-ray, which led to a CT), fear took over, and denial intervened. An unscrupulous traditional medicine practitioner fanned her denial, and promised a cure with Chinese herbs. It could not be cancer, she said. I am healthy and I have never smoked, and besides if it is, I have these herbs. This was a smart woman, a documentary producer, in one of the world’s great cities. There was also the grim reality that to prove it was cancer would involve hospitalization and a grisly procedure, called transthoracic needle aspiration, that would in all likelihood collapse her lung and would in all certainty be agonizing. Except for giving birth, she had never been hospitalized. She was warned by me that she had, at best, 18 months to live if she refused treatment, but that resection would likely cure her. She refused. Her brilliant unconscious was not subject to the rules of transparency and accountability that her brilliant consciousness used, and it slipped off into the dark; it did its calculus and demanded denial as a plan of action. She could not cope with the dissonant truth of cancer. 17 months later, she became quite confused…from brain metastases. One month later she died. Her children are still at home, and motherless. And yet had Biocept’s system been available, a mere blood draw could have provided proof positive of lung cancer, and guided therapy (surgery, drugs, radiation, or what combination). Had Biocept’s method been available, she might now be alive: the data would have convinced her she had cancer that was serious and invasive.

A fascinating just-published study in Nature Medicine, unrelated to Biocept’s efforts, raises the possibility that methods to catch circulating tumor cells are so good now that such cells might be propagated outside the body, in culture or in mice, and then characterized, DNA-assessed, in order to predict response to various interventions. The cells could isolated from a tube of your blood, placed in a mouse, allowed to grow, then harvested and their DNA deep-sequenced.

(2) Will Biocept’s method ever become a screening test for cancer? Many medical professionals reading this may recall a now-nearly-forgotten New England Journal of Medicine paper from when I was in medical school that showed a difference in nuclear magnetic resonance spectoscopy signal between blood of people with and without cancer. The test would give no clue as to type of cancer, of course, and neither could it necessarily be reliable for picking up early stage disease. We will continue to need to screen high risk organs, such as breast, prostate, and colon. Japanese people in Japan will still need screening upper endoscopies at age 40 because of the high risk of gastric cancer in that setting.

Will it ever become a routine blood test to look for cancer? I suspect it will not be priced to make that affordable, and not covered by payers, but if a patient is having other symptoms or labs suggestive of undiagnosed malignancy, it may be considered. Certainly given the breadth and specificity of the testing antibodies Biocept has, it is reasonable to predict that from the blood test, it could narrow down what organ is involved in the cancer.

Again comes the question, however, of whether it will replace biopsy. If the test picked up a cell likely from the pancreas, and the patient has a radiographic pancreas mass, at present oncologists would refuse to treat that patient without tissue from the pancreas lesion showing cancer. With time, with proof of Biocept’s method, with oncology getting farther down the learning curve of this method, however, that may change, especially at academic centers.

(3) If I have cancer, have had a biopsy, and am receiving treatment, does Biocept’s method have anything to offer me? It very much does. Tumor behavior, tumor oncogene expression can change with time, on the basis of what drugs have been given. Those drugs may kill off sensitive tumor parts, and select for resistant cells. Biocept allows determination of this without another biopsy.

The time may come, depending upon price, when circulating tumor cell burden is used as a way to follow response to chemotherapy, much as repeat CT to measure lesion size now is. This fascinating full-text article from 2010 suggests that burden of circulating tumor cells alone is an excellent independent prognostic marker for outcomes in colon, breast and prostate cancer.

Take a good hard look at Biocept, and consider putting some shares in the speculative part of your portfolio. Aegis Capital recently initiated coverage with a buy rating, and a price target of $16, a far cry from the recent $5 share price. Have a go, as I know you will, at Biocept’s recent corporate presentation, where certain particulars are explained in further, if drier, detail. Biocept is certainly the most exciting medical diagnostics company I have ever encountered, and will probably shake up the cancer diagnosis and treatment paradigm like nothing else will. Biocept finds needles in haystacks, but a company this novel, with technology this exceptional, poised for the sort of growth it seems destined for, makes this company a needle-in-a-haystack itself.

This is a discussion topic or guest posting submitted by a Stock Gumshoe reader. The content has not been edited or reviewed by Stock Gumshoe, and any opinions expressed are those of the author alone.

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Travis Johnson, Stock Gumshoe
June 12, 2014 11:02 am

I don’t know anything about this company, other than what I read in Dr. KSS’s excellent commentary above, but yes, it’s a good reminder that if a stock has trading volume of only $100,000 on a typical day then any attention at all can drive it bonkers. When this happens to stocks because of a big newsletter teaser campaign the stock typically drifts back down after a few weeks when the attention dies off, but who knows, “typically” doesn’t really apply for $25 million nanocaps.

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dianagrace11
dianagrace11
June 12, 2014 11:21 am

Just read a story about a woman who had fibroids removed by a procedure called laparoscopic power morcellation – which cuts the fibroids into little pieces to be removed through tiny incision. Fibroids are usually benign of course so I guess no one thought to check but it turned out she had cancer. Had she had BIOC’s test for CTC’s, they’d have known she had cancer and not shredded all those fibroids which caused the cancer to spread. Morcellation is supposed to contain the shredded material in a surrounding bag but that didn’t work, in this case.
Dr. KSS not only gave us a great stock tip but God forbid, if you or a loved one has cancer, we all now know wherever the cancer occurs – take it out whole – don’t shred it for the sake of a small incision.

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Gary Reiter
June 12, 2014 7:32 pm
Reply to  dianagrace11

From the FDA, released April 17, 2014
http://www.fda.gov/newsEvents/Newsroom/PressAnnouncements/ucm393689.htm

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justirregular
June 12, 2014 12:56 pm

Dr, KSS, Thank You again for an extremely educational read. Now I have to get a couple more books thanks to you! 😉

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Alan Harris
Guest
Alan Harris
June 12, 2014 5:18 pm

Sorry guys but the footie has started (for some odd reason you call it soccer???) All bets are off till the final whistle blows.
Forget all that ‘Men in armour plated suits’ stuff….. This is a man’s game. No tin helmets.

Travis Johnson, Stock Gumshoe
June 12, 2014 7:10 pm
Reply to  Alan Harris

Crazy game… started out hoping for some acrobatics from Brazil, but Croatia won me over — too bad they got the short end from lady fortune and the guy with the whistle. Oh, and their goalie.

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herbalix
Member
June 13, 2014 12:00 pm

Absolutely agree Travis. The longer the game went on the more I wished Croatia would have won the game! At least a tie would have been in order! I do not believe their goalie had anything to do with the loss. The sad thing about the soccer is that not only the better team wins. lady fortune clearly had made up her mind before.

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herbalix
Member
June 13, 2014 11:53 am
Reply to  Alan Harris

I am with you on that one Allan. Ready to watch some good games for the next few weeks! The only thing that i do hate is when guys are acting to get penalty shots or other free-shots and that they act like they are dying when they get hardly get touched by the other guy. Then they waste lots of time lying around on the grass afterwards they run around as if nothing ever happened to them. Other than that my favorite game in the whole world!!! it is on in five minutes. hear you later…Have fun!

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Alan Harris
Guest
Alan Harris
June 13, 2014 1:56 pm
Reply to  herbalix

What you have to remember is that the referee is a representative of the crowd. We all see it differently (until video replay) That difference of view point is what we fight about down at the pub…….. That’s where the adrenaline is in the game. Its a ‘no contact sport’, so they fall over when theres no contact 🙂 . Its a GAME within a game.

Holland vs Spain tonight….should be a goodie !!

Alan Harris
Guest
Alan Harris
June 13, 2014 4:58 pm
Reply to  herbalix

Oh, OMG, OMG, OMG, OMG, OMG 5-1. it could have been 8-1. I couldnt be happier if Beni hit $5. If the final is THAT exciting, it’ll be a joy. Go Holland….go Belgium. and of course GO ENGLAND.

Subramania Kaushik
Subramania Kaushik
June 13, 2014 5:03 pm
Reply to  Alan Harris

Holland destroyed Spain. Just can’t believe it!

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Opposeablethumb
Irregular
June 12, 2014 5:22 pm

Many moons ago Flamel Technologies (FLML) was on my list of nano-tech companies. Bought high sold low cuz I was a Fool.

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eyedoc
June 12, 2014 7:35 pm

fool, not Fool.

bobbyb444
bobbyb444
June 12, 2014 6:46 pm

Good informative article, even though I did not fully understand some of the medical terms…
However, I wonder if this BIOC test is/was a precursor to, or an identical test of, the newest company on the market, called the “OncoBlast” test, which also tests one’s blood for cancer markers, for over 20 known cancers:

“The ONCOblot® Test can detect cancer before clinical symptoms appear. The ENOX2 protein species in the blood is unique to malignant cells and these proteins can be detected in early development….”

(see http://www.oncoblotlabs.com/)

Curious if they are affiliated??

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bobbyb444
bobbyb444
June 12, 2014 6:48 pm

OncoBlast test costs about $1,100 +/-

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chibana
chibana
June 12, 2014 7:17 pm

Dr. Kss,
Thanks again for another great article. I picked up some shares at $5.44.
V/R
Tom

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Ogle
Ogle
June 12, 2014 10:05 pm

Again the most interesting articles anyone could write about bio companies and the biology behind the workings of the their treatments. DR KISS you are one of a kind, and I thank you.
DRTX is correcting back down to the top of it’s gap up on May 12…..remember most gaps eventually are filled and this one looks like it is underway. I sold as it approached the double top on May 19 and will buy it back at 14.50 or so, as it sinks to the top of the gap range. The stock is behaving as it should as market makers and traders sold out on the technical double top pattern. Rinse and repeat is the rule. Today’s long red candle with a long wick hanging down shows buying interest at that level but it broke down through it’s 20 day exp. moving average, and that is not a good sign. May have more downside to the 14.45 gap support.
As for BIOC, today was not the best day to take a position in this thinly traded stock with all us Gummies buying. Patience will bring us a price in the mid 4.00 range where it spent most of the last six weeks prior to the KSS article and increased buying. After buying at 4.50 ish I expect that the price level around 5.80 – 5.90 to be a difficult area to break through as it has failed three times now to do that. I will sell at least 1/2 of the position just under 5.80 for a 25% gain if it allows the 4.50 ish entry, and hold the remainder to see what it does with that resistance level. If it reverses again, it’s rinse and repeat or, if it breaks out it’s reload the 1/2 position. Greatly reduces the risk of buy and hold on such a thinly traded stock. Capital is King and we must preserve it to trade a better potential. My mental stop for BIOC is a price below 4.31 in the final five minutes of the market, so downside risk from 4.50 is 0.19 and target of 5.76 gives a reward of 1.26 for a risk / reward ratio of 1:6.6. Very good odds for a 25% gain potential. Sooner or later, news will cause the stock to rise or fall in an impressive manner and that signals the time to think about longer time holding or walking away for good.
Walter
The past two days were for enjoying the fruits of holding NWBO for several years now with good news coming from the patient trials. This stock should already be over 15.00 absent the huge shorting by the manipulators associated with The Street and AF. When the six million shares shorted give up and cover it will explode IMHO. The only risk remaining in NWBO is the question of duration of the treatment and all indications I have read from prior testing in primates point to long term cure. Chemo and radiation may fade away as the SOC.

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clomu
clomu
June 12, 2014 10:25 pm
Reply to  Ogle

Thanks Walter, appreciate the info.

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isabelle
isabelle
June 12, 2014 11:36 pm
Reply to  Ogle

Thank you for the information, Walter. I always learn so much from you!

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Alan Harris
Guest
Alan Harris
June 13, 2014 4:03 am
Reply to  Ogle

Nicely written and well thought through. Thanks

analog68
analog68
June 13, 2014 7:11 am
Reply to  Ogle

Always spot on Walter, keep them coming.

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bwd1up
bwd1up
June 13, 2014 10:54 am
Reply to  Ogle

Thank you Walter, very informative!

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tanglewood
June 13, 2014 11:26 am
Reply to  Ogle

hi Walter, what are the tea leaves showing for OXBT?

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jyoung0071
jyoung0071
June 20, 2014 2:25 pm
Reply to  Ogle

If you are saying AF is a “manipulator” and associated in a concerted manipulative way with the shorts, I must say that I disagree completely. Imo his thinking is as good as it gets. He presents both the bull side and the bear side. Seems a bunch of people detest hearing the bear side.

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tomtucker
June 12, 2014 10:38 pm

Dr KSS ,
Way back on a thread of long ago we discussed SNTI and why it ‘s SP has been off of late and
I promised a link to their presentation at ASCO .
Well, after a lesson from Travis’s girl Friday, I am able to post the link which follows:
http://abstracts.asco.org/144/AbstView_144_129505.html

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David B
Guest
June 13, 2014 2:07 am

Doc–have you commented on Conatus Pharma before? It’s in your wheelhouse (liver disease) so I’m assuming you are not a fan as the name didn’t ring a bell for me. New article on Conatus on SA and apparently the stock has about 80% institutional investors so a lot of supposedly smart money in this one. It’s also had a big move up in recent days for some reason.

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David B
Guest
June 13, 2014 2:13 am

DoctorJack on another thread is talking smack about my beloved SVA–how dare he! It hasn’t done great in recent months (along with 90% of small biotech stocks), but for a patient investor it will be a very good investment over time. Also, don’t worry about Durata–be patient with this one as well. The way I see it, folks pay way too much attention to short term movements. Unless you are great at interpreting stock movement like Walter, don’t get too caught up in this or you will lose your shirt.

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beluga
beluga
June 13, 2014 11:22 pm
Reply to  David B

Director of Investor Relations, Helen Yang, discusses Sinovac’s (NASDAQ: SVA) future opportunities.
http://www.benzinga.com/general/topics/14/06/4630900/exclusive-sinovac-ir-director-explains-massive-revenue-opportunity

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David B.
David B.
June 13, 2014 11:49 pm
Reply to  beluga

Nice link Monika K Sinovac is the real deal. I will be very patient with this one and patience will pay off.

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tomtucker
June 13, 2014 6:48 am

Dr KSS,
Sorry I failed to send the actual poster that Senesco(Snti) presented at ASCO.
You can find it here.
: Poster – TPS8616, ASCO, 06-02-2014.pdf
I know you said at one time that this was a short time play. Any pertinent info in this poster?

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tomtucker
June 13, 2014 7:00 am

Here’s the whole message I got from SNTI. Maybe the poster will down load from that.

Attachments: Poster – TPS8616, ASCO, 06-02-2014.pdf
The following information is from the press release. I have also attached a copy of the poster. Follow the link below for additional information. Let me know, if you would like to be added to the Blast List and receive future releases.

Senesco Announces Update on SNS01-T at ASCO
Presentation at the 50th Annual Meeting of the American Society of Clinical Oncology

BRIDGEWATER, N.J. (June 2, 2014) – Senesco Technologies, Inc. (“Senesco” or the “Company”) (OTC QB: SNTI), announced that Alice Bexon, M.D., Senesco’s Vice President of Clinical Development, will present a poster at the 50th Annual Meeting of the American Society of Clinical Oncology in Chicago, Illinois.
Poster Session, McCormick Place, Chicago, IL (May 30 – June 3, 2014)
Date/Time: Monday, June 2, 2014; 1:15 PM to 5:00 PM (CT)
Location: S Hall A2, Poster Board: 300b
Session: Lymphoma and Plasma Cell Disorders
Poster Number: TPS8616: “Phase 1-2 open-label, multiple-dose, dose-escalation study to evaluate the safety and tolerability of intravenous infusion of SNS01-T, a first-in-class modulator of eukaryotic translation initiation factor 5A (eIF5A) in patients (pts) with relapsed or refractory B-cell malignancies.” http://abstracts.asco.org/144/AbstView_144_129505.html

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Travis Johnson, Stock Gumshoe
June 13, 2014 8:26 am
Reply to  tomtucker

Hi Thomas — looks like the pdf was in an email attachment to you, which means it’s not a file on the web that you can link to and I didn’t see it available online in my quick glance. If you send us the email with attachment at payments.com and note they’re for Dr. KSS we can forward the message.

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capndave040
capndave040
June 13, 2014 8:32 am

Thanks to all for another great educational, and hopefully, profitable experience. Dr. K your information is priceless. Nowhere else could I get this knowledge.
I do wish however, that all of us would remember, as Walter and Elliott so clearly point out, is that there is also a “science” involved in buying and selling stocks as well. The technical formations that repeat over and over again in the market must be acknowledged or the piper will be paid. Double tops and bottoms, short sells and covers, volume trends and spikes, and perhaps most significantly with “nano” stocks like these, the significance of “gaps” have to be considered when decisions on entry and exits are decided. Writing a “market” order for any stock, especially these, seems to me to be an open invitation to be “taken.” Learning to use limit orders, and Good Until Cancelled (GTC orders) will reinforce the discipline and patience needed to be a successful investor.
When I was in the business the saying was “Limits limit profits.” That’s true, they limit profits for the broker, they protect the investor. Gaps will close, bottoms and tops will be tested. “Wash, rinse, repeat” Walter wisely states. Please friends, (and although we are all competitors in this world of investing, I do feel that we are friends), pay attention to the technical aspects as well as the science. Let’s not buy without DD, including “technical” DD. Let’s not give the market makers control. We are the bid side, don’t believe you automatically have to buy on the “ask.”
For those of us that are just starting out I suggest that learning to interpret red/green candlestick charts is a great place to begin.
What an exciting time to be alive.

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Elliot
June 13, 2014 9:04 am
Reply to  capndave040

nice post, Dave

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clomu
clomu
June 13, 2014 10:01 am

NKTR is racing up today.

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Alan Harris
Guest
Alan Harris
June 13, 2014 1:12 pm
Reply to  clomu

It got a free pass thru phase 3 from advisory committee.

newbie
Member
June 13, 2014 10:12 am

Anyone know what the support level of DRTX is ? It hit 14.98 last month…
Thanks. JB

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Elliot
June 13, 2014 10:19 am

I’m no expert, Jana, but from a quick look at some charts, it looks like there is major support at 13.20ish (bottom of uptrend channel) and then more support around 12.00. I’m taking that info from finviz http://finviz.com/quote.ashx?t=drtx&ty=c&ta=1&p=d
I also use stock tracker to track some moving averages (which can also be done in yahoo! finance using the “interactive” tool on the left). From there, you can see the 50 day sma is at 14.49 right now, so this should be a pretty good support level also. 100 day sma is at 13.89, and the 200 day sma is at 12.09. So there are quite a few support levels, the first being 14.49 (50dma), then 13.89 (100dma), then 13.20 (bottom of uptrend channel), then 12.09 (200dma), then 12.00 (historical support area).

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newbie
Member
June 13, 2014 10:23 am
Reply to  Elliot

Thanks Elliot!

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Elliot
June 13, 2014 10:41 am
Reply to  pm1773

yeah, I tend to think these are just pulled out of thin air. They don’t know what’s going on, so they come up with whatever explanation they can think of. I think this happens a lot with stock market coverage.

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Elliot
June 13, 2014 10:57 am

Some Navidea Bio (NAVB) today. I feel this company has similar interests to BIOC, though they are identifying lymph nodes, not free floating cancer cells that could be anywhere in the blood. I don’t know enough about NAVB or BIOC to comment on which approach is more promising. It seems both may have their advantages and disadvantages. Nevertheless, NAVB’s market cap may be a good point of reference for BIOC investors? I’m not sure, but it seems NAVB is not much further along than BIOC, although they do have more products. Lymphoseek did not have any sales in 2012.

Label expansion for Lymphoseek • 10:48 AM
•The FDA approves the use of Navidea Biopharmaceuticals’ (NAVB -7.4%) radioactive imaging agent Lymphoseek for use in sentinel lymph node biopsies. Sentinel lymph nodes are those closest to a primary tumor for cancer. This new indication allows for more limited lymph node surgery in patients with sentinel nodes negative for cancer.
•Last year, the regulator cleared the product as an aide in the identification of lymph nodes closest to a primary tumor in patients with breast cancer or melanoma.

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Elliot
June 13, 2014 10:58 am
Reply to  Elliot

that said, NAVB seems ridiculously overpriced at 150x sales. So maybe its market cap is far far too high, and BIOC’s market cap is more reasonable.

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Elliot
June 13, 2014 10:59 am
Reply to  Elliot

correction: some NAVB *news out* today ^. I left a couple of words out there

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